CS241506B2 - Sensitized agent in form of particles for immunological reactions - Google Patents
Sensitized agent in form of particles for immunological reactions Download PDFInfo
- Publication number
- CS241506B2 CS241506B2 CS823120A CS312082A CS241506B2 CS 241506 B2 CS241506 B2 CS 241506B2 CS 823120 A CS823120 A CS 823120A CS 312082 A CS312082 A CS 312082A CS 241506 B2 CS241506 B2 CS 241506B2
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- sensitized
- antibody
- particles
- buffer
- reagent
- Prior art date
Links
- 239000002245 particle Substances 0.000 title claims description 34
- 230000008105 immune reaction Effects 0.000 title abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 29
- 239000000427 antigen Substances 0.000 claims abstract description 26
- 102000036639 antigens Human genes 0.000 claims abstract description 26
- 108091007433 antigens Proteins 0.000 claims abstract description 26
- 239000012736 aqueous medium Substances 0.000 claims abstract description 12
- 239000000969 carrier Substances 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 230000001900 immune effect Effects 0.000 claims description 2
- 230000004048 modification Effects 0.000 claims description 2
- 238000012986 modification Methods 0.000 claims description 2
- 239000000872 buffer Substances 0.000 description 27
- 229920000126 latex Polymers 0.000 description 16
- 239000004816 latex Substances 0.000 description 15
- 230000035945 sensitivity Effects 0.000 description 11
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 10
- 239000000126 substance Substances 0.000 description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 7
- 229940098773 bovine serum albumin Drugs 0.000 description 7
- 239000004471 Glycine Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 239000003381 stabilizer Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 6
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 239000007983 Tris buffer Substances 0.000 description 5
- 239000007864 aqueous solution Substances 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 229940049920 malate Drugs 0.000 description 5
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 4
- 230000004520 agglutination Effects 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000003755 preservative agent Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- -1 pollen Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000009257 reactivity Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- RNMCCPMYXUKHAZ-UHFFFAOYSA-N 2-[3,3-diamino-1,2,2-tris(carboxymethyl)cyclohexyl]acetic acid Chemical compound NC1(N)CCCC(CC(O)=O)(CC(O)=O)C1(CC(O)=O)CC(O)=O RNMCCPMYXUKHAZ-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- NLHHRLWOUZZQLW-UHFFFAOYSA-N Acrylonitrile Chemical compound C=CC#N NLHHRLWOUZZQLW-UHFFFAOYSA-N 0.000 description 1
- 108010074051 C-Reactive Protein Proteins 0.000 description 1
- 102100032752 C-reactive protein Human genes 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 241000606701 Rickettsia Species 0.000 description 1
- 206010070834 Sensitisation Diseases 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 241000295644 Staphylococcaceae Species 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 125000003368 amide group Chemical group 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 239000007982 barbital buffer Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000013024 dilution buffer Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 108010074605 gamma-Globulins Proteins 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229940106780 human fibrinogen Drugs 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 239000006174 pH buffer Substances 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 229920003048 styrene butadiene rubber Polymers 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229960001322 trypsin Drugs 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Sampling And Sample Adjustment (AREA)
- Conductive Materials (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
- Silicon Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56067332A JPS57182168A (en) | 1981-05-02 | 1981-05-02 | Immunochemical reagent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS312082A2 CS312082A2 (en) | 1985-08-15 |
| CS241506B2 true CS241506B2 (en) | 1986-03-13 |
Family
ID=13341952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS823120A CS241506B2 (en) | 1981-05-02 | 1982-04-30 | Sensitized agent in form of particles for immunological reactions |
Country Status (17)
| Country | Link |
|---|---|
| EP (1) | EP0064275B1 (en]) |
| JP (1) | JPS57182168A (en]) |
| KR (1) | KR900002347B1 (en]) |
| AT (1) | ATE15107T1 (en]) |
| AU (1) | AU550925B2 (en]) |
| CA (1) | CA1186222A (en]) |
| CS (1) | CS241506B2 (en]) |
| DD (1) | DD204313A5 (en]) |
| DE (1) | DE3265562D1 (en]) |
| DK (1) | DK157109C (en]) |
| ES (1) | ES511798A0 (en]) |
| HU (1) | HU186928B (en]) |
| IL (1) | IL65648A0 (en]) |
| NO (1) | NO159964C (en]) |
| PT (1) | PT74834B (en]) |
| SU (1) | SU1431662A3 (en]) |
| ZA (1) | ZA822727B (en]) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59183369A (ja) * | 1983-04-01 | 1984-10-18 | Kazue Ueno | ラテツクス試薬 |
| JPS6035263A (ja) * | 1983-08-05 | 1985-02-23 | Wako Pure Chem Ind Ltd | 不溶性担体に固定化された免疫活性物質の安定化法及び該物質を構成単位として含む生理活性物質測定用試薬 |
| US4713350A (en) * | 1983-10-24 | 1987-12-15 | Technicon Instruments Corporation | Hydrophilic assay reagent containing one member of specific binding pair |
| FR2589239B1 (fr) * | 1985-10-24 | 1989-10-20 | Cottingham Hugh | Chambre de reaction agglutinographique |
| EP0280559B1 (en) * | 1987-02-27 | 1993-10-20 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Agglutination immunoassay and kit for determination of a multivalent immune species using a buffered salt wash solution |
| RU2298798C1 (ru) * | 2006-01-17 | 2007-05-10 | Общество с ограниченной ответственностью "Биочип-Аналитика" (ООО "Биочип-Аналитика") | Способ контроля биологической пробы в реакции латекс-агглютинации и аналитическая система для его осуществления |
| JP4486059B2 (ja) * | 2006-05-25 | 2010-06-23 | デンカ生研株式会社 | 免疫測定用ラテックス組成物 |
| WO2010101213A1 (ja) | 2009-03-05 | 2010-09-10 | デンカ生研株式会社 | 検査試薬及びそれを用いた被検試料中の被測定物の測定方法 |
| ES2685332T3 (es) * | 2011-12-13 | 2018-10-08 | Baxalta GmbH | Medición de autoanticuerpos en condiciones de baja conductividad |
| US12247987B2 (en) | 2018-07-13 | 2025-03-11 | Siemens Healthcare Diagnostics Inc. | Methods for detection of aberrant results caused by incomplete dispersion of immunoassay reagent |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1362776A (en) * | 1970-07-17 | 1974-08-07 | Wellcome Found | Immunological reagent |
| US3857931A (en) * | 1971-02-01 | 1974-12-31 | Hoffmann La Roche | Latex polymer reagents for diagnostic tests |
| JPS5811575B2 (ja) * | 1976-08-16 | 1983-03-03 | 帝国臓器製薬株式会社 | 抗原−抗体反応の測定法 |
| US4092114A (en) * | 1976-10-20 | 1978-05-30 | Fisher Scientific Company | Indirect latex test for determination of immunoglobulins |
| IT1087285B (it) * | 1976-11-10 | 1985-06-04 | Hoffmann La Roche | Procedimento di determinazione immunologico |
| FR2399448A1 (fr) * | 1977-08-03 | 1979-03-02 | Rhone Poulenc Ind | Latex de polymeres vinyliques stables aux electrolytes |
| EP0001223A3 (en) * | 1977-09-19 | 1979-12-12 | F.Hoffmann-La Roche & Co. Aktiengesellschaft | Latex coated with a polyhydroxy compound, process for the preparation of this latex, immunological reagent containing this latex, process for the preparation of this reagent, application of this reagent, testing procedure utilising this reagent and reagent kit containing this reagent |
| GB2013211B (en) * | 1978-01-26 | 1982-06-30 | Technicon Instr | Immunoassays using f(ab')2 fragments |
| GB2027031A (en) * | 1978-08-02 | 1980-02-13 | Hoffmann La Roche | Diagnostic Reagent Comprising a Proteinaceous Material Bonded to a Latex |
-
1981
- 1981-05-02 JP JP56067332A patent/JPS57182168A/ja active Granted
-
1982
- 1982-04-19 AU AU82824/82A patent/AU550925B2/en not_active Expired
- 1982-04-21 ZA ZA822727A patent/ZA822727B/xx unknown
- 1982-04-28 EP EP82103640A patent/EP0064275B1/en not_active Expired
- 1982-04-28 DK DK190582A patent/DK157109C/da not_active IP Right Cessation
- 1982-04-28 DE DE8282103640T patent/DE3265562D1/de not_active Expired
- 1982-04-28 AT AT82103640T patent/ATE15107T1/de not_active IP Right Cessation
- 1982-04-29 ES ES511798A patent/ES511798A0/es active Granted
- 1982-04-29 IL IL65648A patent/IL65648A0/xx not_active IP Right Cessation
- 1982-04-30 HU HU821360A patent/HU186928B/hu not_active IP Right Cessation
- 1982-04-30 NO NO821448A patent/NO159964C/no unknown
- 1982-04-30 PT PT74834A patent/PT74834B/pt not_active IP Right Cessation
- 1982-04-30 KR KR8201912A patent/KR900002347B1/ko not_active Expired
- 1982-04-30 DD DD82239479A patent/DD204313A5/de not_active IP Right Cessation
- 1982-04-30 CS CS823120A patent/CS241506B2/cs unknown
- 1982-05-03 SU SU823430146A patent/SU1431662A3/ru active
- 1982-05-03 CA CA000402148A patent/CA1186222A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| PT74834A (en) | 1982-05-01 |
| JPS6367865B2 (en]) | 1988-12-27 |
| AU8282482A (en) | 1982-11-11 |
| ZA822727B (en) | 1983-11-30 |
| SU1431662A3 (ru) | 1988-10-15 |
| NO159964B (no) | 1988-11-14 |
| EP0064275A1 (en) | 1982-11-10 |
| NO821448L (no) | 1982-11-03 |
| HU186928B (en) | 1985-10-28 |
| CA1186222A (en) | 1985-04-30 |
| KR900002347B1 (ko) | 1990-04-12 |
| EP0064275B1 (en) | 1985-08-21 |
| AU550925B2 (en) | 1986-04-10 |
| JPS57182168A (en) | 1982-11-09 |
| KR830010385A (ko) | 1983-12-30 |
| NO159964C (no) | 1989-02-22 |
| DD204313A5 (de) | 1983-11-23 |
| ES8401260A1 (es) | 1983-12-01 |
| ATE15107T1 (de) | 1985-09-15 |
| CS312082A2 (en) | 1985-08-15 |
| IL65648A0 (en) | 1982-07-30 |
| DK190582A (da) | 1982-11-03 |
| DK157109C (da) | 1990-04-09 |
| DK157109B (da) | 1989-11-06 |
| ES511798A0 (es) | 1983-12-01 |
| DE3265562D1 (en) | 1985-09-26 |
| PT74834B (en) | 1983-10-28 |
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